Journal: Journal of Neuroinflammation

Article Title: Astroglia acquires a toxic neuroinflammatory role in response to the cerebrospinal fluid from amyotrophic lateral sclerosis patients

doi: 10.1186/s12974-016-0698-0

Figure Lengend Snippet: List of the antibodies used in the study

Article Snippet: Anti-GDNF mouse monoclonal (1:200, SCBT), 24 h, 4 °C , Anti-mouse IgG (Cy3-conjugated; 1:200, Sigma-Aldrich) 2 h, RT.

Techniques:

Journal: Neurotherapeutics

Article Title: Glial Cell Line–Derived Neurotrophic Factor and Chondroitinase Promote Axonal Regeneration in a Chronic Denervation Animal Model

doi: 10.1007/s13311-019-00745-0

Figure Lengend Snippet: Immunofluorescence antibodies used for the in vivo release kinetics and mechanistic studies

Article Snippet: Primary , Rabbit anti-GDNF , PeproTech , 500-P81 , 1:500.

Techniques: Immunofluorescence, In Vivo, Plasmid Preparation

Journal: PLoS ONE

Article Title: Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF

doi: 10.1371/journal.pone.0212991

Figure Lengend Snippet: A: The expression of mRNA for Ret, GFR-alpha 1, GFR-alpha 2, GFR-alpha 3, and GFR-alpha 4 in RPTECs cultured in a monolayer were examined with RT-PCR (n = 3). Representative images are shown. B: Expression of Ret in RPTECs cultured in a monolayer for 5 days or cultured in gels for 8 days. Ret (green), DAPI (blue). C: The expression of mRNA for GDNF, NRTN, ARTN, and PSPN in HUVECs cultured in a monolayer was examined with RT-PCR (n = 3). D: Production of GDNF in HUVECs (n = 4) or RPTEC (n = 4) cultured in a monolayer was examined with western blot analysis. P, positive control (rat brain).

Article Snippet: Antibodies used in this study were as follows: goat polyclonal anti-GDNF antibody (AF-212-NA) (R&D Systems); mouse anti-beta-actin antibody (3598R-100) (BioVision, Milpitas, CA); rabbit monoclonal anti-Ret antibody (E1N8X) (CST #14556) (Cell Signaling Technology, Danvers, MA); rabbit monoclonal anti-Ret antibody (ab134100) (Abcam, Cambridge, MA); anti-Met antibody (ab39075) (Abcam, Cambridge, MA); anti-phospho-Met antibody (CST #3077) (Cell Signaling Technology, Danvers, CO); anti-aquaporin 1 (AQP1) antibody (sc-20810) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Expressing, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot, Positive Control

Journal: PLoS ONE

Article Title: Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF

doi: 10.1371/journal.pone.0212991

Figure Lengend Snippet: A: Morphology of RPTECs cultured in gels in the absence or presence of HGF (50 ng/ml) with GDNF (100 ng/ml) for 5 days (Magnification, ×100 and ×400). Arrowheads indicate the lumen of tubular structures. B: Quantitative analysis of tubule formation. RPTECs were cultured in gels with the indicated factors for 5 days. Values are the mean ± SE (n = 3). *P < 0.05. C: Quantitative analysis of branch number per tubular structure. Values are the mean ± SE (n = 5).

Article Snippet: Antibodies used in this study were as follows: goat polyclonal anti-GDNF antibody (AF-212-NA) (R&D Systems); mouse anti-beta-actin antibody (3598R-100) (BioVision, Milpitas, CA); rabbit monoclonal anti-Ret antibody (E1N8X) (CST #14556) (Cell Signaling Technology, Danvers, MA); rabbit monoclonal anti-Ret antibody (ab134100) (Abcam, Cambridge, MA); anti-Met antibody (ab39075) (Abcam, Cambridge, MA); anti-phospho-Met antibody (CST #3077) (Cell Signaling Technology, Danvers, CO); anti-aquaporin 1 (AQP1) antibody (sc-20810) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Cell Culture

Journal: PLoS ONE

Article Title: Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF

doi: 10.1371/journal.pone.0212991

Figure Lengend Snippet: A: Production of phospho-Met and total Met protein in RPTECs cultured in a monolayer treated with HGF (50 ng/ml), GDNF (50 ng/ml) or HGF plus GDNF was examined with western blot analysis. Representative results of three independent experiments are shown. B: Quantitative analysis of phospho-Met normalized to total Met. Values are the mean ± SE. *P < 0.05, **P < 0.01 vs. 0 min.

Article Snippet: Antibodies used in this study were as follows: goat polyclonal anti-GDNF antibody (AF-212-NA) (R&D Systems); mouse anti-beta-actin antibody (3598R-100) (BioVision, Milpitas, CA); rabbit monoclonal anti-Ret antibody (E1N8X) (CST #14556) (Cell Signaling Technology, Danvers, MA); rabbit monoclonal anti-Ret antibody (ab134100) (Abcam, Cambridge, MA); anti-Met antibody (ab39075) (Abcam, Cambridge, MA); anti-phospho-Met antibody (CST #3077) (Cell Signaling Technology, Danvers, CO); anti-aquaporin 1 (AQP1) antibody (sc-20810) (Santa Cruz Biotechnology, Dallas, TX).

Techniques: Cell Culture, Western Blot